Storage:
Short-term storage at 4℃, some reagents should be kept at -20℃ when not in use for a long time.
Detection Method:
Colorimetric
Specificity:
This ELISA kit has excellent specificity for the detection of Granzyme H (GZMH).
Assay Type:
Double-antibody sandwich
ELISA Kit Principle:
The ELISA kit uses double-antibody sandwich ELISA to quantitatively detect the target. The microtiter plate has been pre-coated with an antibody specific to Granzyme H (GZMH). Then the standards and samples are added to the plate to react with the biotin-conjugated antibody specific to Granzyme H (GZMH). Next, Avidin conjugated to HRP is added to each well and incubated. After TMB substrate solution is added, only those wells that contain Granzyme H (GZMH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The concentration of Granzyme H (GZMH) in the samples is determined by colorimetric.
Shipping Condition:
Ice packs
Cross Activity:
It has been verified that this product has high specificity and sensitivity to Granzyme H (GZMH) and has no cross activity to other homologs.
Assay Procedure Summary:
1. Prepare the reagents, standards, and samples needed for testing; 2. Add 100 µL/well sample or standard to the microtiter plate, incubate 2 h at 37°C; 3. Aspirate the reagents added in the previous step, and then add 100 µL of the prepared solution A, incubate 1 h at 37°C; 4. After washing the plate 3 times, add 100 µL of the prepared solution B and incubate 30 min at 37°C; 5. After washing the plate 5 times, add 90 µL of Substrate solution and incubate 10-20 min at 37°C; 6. Terminated by the addition of 50 µL stop solution, the absorbance was read at 450 nm by a microplate reader.
Precautions before Use:
The kits of this ELISA kit are not assembled, please assemble it before use.
Stop Solution:
The stop solution of this kit contains sulfuric acid. Please use it safely and do not directly contact it.
Notice:
In order to reduce the influence of various variables on the experimental results during the experiment, you must strictly control the experimental conditions, and the entire experimental operation process should be completed by the same person.
