Storage:
Short-term storage at 4℃, some reagents should be kept at -20℃ when not in use for a long time.
Detection Method:
Colorimetric
Specificity:
This ELISA kit has excellent specificity for the detection of Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- and Steroid Delta-Isomerase 1.
Format:
96-Well Microplate
Shipping Condition:
Ice packs
Standard Form:
Lyophilized
Assay Procedure Summary:
1. Prepare the reagents, standards, and samples needed for testing; 2. Aliquot 50 µl of diluted standard into the standard wells; 3. Aliquot 50 µl of standard diluent buffer into control (zero) well; 4. Add 50 µL/well sample to the microtiter plate; 5. Then add 50 µL of the prepared detection solution A, incubate 1 h at 37°C; 6. After washing the plate 3 times, add 100 µL of the prepared detection solution B and incubate 30 min at 37°C; 7. After washing the plate 5 times, add 90 µL of substrate solution and incubate 10-20 min at 37°C; 8. Terminated by the addition of 50 µL stop solution, the absorbance was read at 450 nm by a microplate reader.
ELISA Kit Components:
Pre-coated 96-Well Microplate
Standard
Solution A
Solution B
Wash Buffer
Detection Solution A
Detection Solution B
Standard Diluent Buffer
TMB Substrate
Stop Solution
Precautions before Use:
The kits of this ELISA kit are not assembled, please assemble it before use.
Stop Solution:
The stop solution of this kit contains acid. Please pay attention to safe use and avoid direct contact.
