Sequence:
MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFQGRRASQ
Activity:
Assay 1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml. Assay 2: The ED50 as determined by a cytotoxicity Assay using HT-29 cells is = 0.05 ng/ml, corresponding to a specific activity of = 2 x 10^7 units/mg.
Endotoxin:
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
Purity:
Greater than 98% by SDS-PAGE and HPLC
Reconstitution:
Reconstitute in distilled water to a concentration of 1.0 mg/mL
Storage:
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
