Sequence:
SHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKHLGIEFMEAE
Activity:
The ED50 as determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is <=0.5 ng/ml, corresponding to a specific activity of >=2 x 10^6 units/mg.
Endotoxin:
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
Purity:
Greater than 98% by SDS-PAGE and HPLC
Reconstitution:
Centrifuge the vial before opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week.
Storage:
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
