Human Phospho-CDK1/2 (Thr14) Ready-To-Use IHC Kit

Catalog No:

RTUI0356

Sample Type:

FFPE tissue

Storage:

Please store components at the temperatures indicated on the individual tubelabels.The kit is stable for 6 months from the date of receipt.

Size:

50T (including a control slide)

Notes:

The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
Do not allow slides to dry at any time during this procedure.
Please don't replace the matching reagents in this product with other manufacturers' products.
As DAB is a carcinogen, please take necessary precautions.
PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.

Synonyms:

CDC2, Cdc2a, CDK1, p34, Cdc 2, Cdc2, CDC28A, CDK 1, CDK-1, CDK1_HUMAN, CDKN1, CELL CYCLE CONTROLLER CDC2, Cell division control protein 2, Cell division control protein 2 homolog, Cell division cycle 2 G1 to S and G2 to M, Cell division protein kinase 1, Cell Divsion Cycle 2 Protein, Cyclin Dependent Kinase 1, Cyclin-dependent kinase 1, DKFZp686L20222, MGC111195, p34 Cdk1, p34 protein kinase, P34CDC2.

Introduction:

CDK1 or Cell Division Control protein 1 is essential for the completion of START, the controlling event in the cell cycle that is required to initiate mitosis. CDK1 is a catalytic subunit of a protein kinase complex, called the M-Phase Promoting Factor that induces entry into mitosis and is universal among eukaryotes. Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays the onset of apoptosis. Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.