Human ZAP70 Ready-To-Use IHC Kit

Catalog No:

RTUI0484

Product Name:

Human ZAP70 Ready-To-Use IHC Kit

Sample Type:

FFPE tissue

Storage:

Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt.

Size:

50T (including a control slide)

Notes:

The positive control slide provided in the kit allows you to be sure that the experimental set-up is working properly.
Do not allow slides to dry at any time during this procedure.
Please don't replace the matching reagents in this product with other manufacturers' products.
As DAB is a carcinogen, please take necessary precautions.
PBS (reagent 1) can be stored for one week at 4℃ after preparation; The antigen retrieval buffer (1×reagent 2) and the chromogenic agent (the mixture of reagents 7 and 8) should be prepared right before each assay.

Synonyms:

ADMIO2; IMD48; mrtle; mur; SRK; STCD; STD; TZK; ZAP-70; ZAP70.

Introduction:

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.