Warn:
The stop solution contains dilute sulfuric acid, please do not touch it directly.
ELISA Kit Principle:
The ELISA kit uses indirect ELISA to quantitatively detect IgG4 antibodies. The IgG4 antibodies of the serum and the immobilized antigens will be bound to pre-coated 20 different food antigens in the plate. After incubation and washing at a suitable temperature and a certain period of time, the anti-human-IgG4-AP conjugate is added, and then TMD is added for the color reaction and the result is measured at 405 nm. The intensity of the color in the microplate is positively correlated with the concentration of the analyte.
Cross Activity:
This ELISA kit specifically detects IgG4 Antibodies Against 20 Food Allergens and almost has no cross-reactivity to IgE.
Storage:
The ELISA kit should be stored at 2-8°C and should be performed at room temperature when used.
Notice:
In order to reduce the influence of various variables on the experimental results during the experiment, you must strictly control the experimental conditions, and the entire experimental operation process should be completed by the same person.
Precautions before Use:
Prepare the conjugate, substrate, buffer and stop solution. And before the test analysis, the reagents in the ELISA kit and the sample to be tested need to be incubated to room temperature.
Detection Method:
Colorimetric
Shipping Condition:
Ice packs
