Sequence:
MHKSSPQGPDRLLIRLRHLIDIVEQLKIYENDLDPELLSAPQDVKGHCEHAAFACFQKAKLKPSNPGNNKTFIIDLVAQLRRRLPARRGGKKQKHIAKCPSCDSYEKRTPKEFLERLKWLLQKMIHQHLS
Activity:
Determined by its ability to stimulate the proliferation of human ANBL-6 cells. The expected ED50 is = 1.0 ng/ml, corresponding to a specific activity of = 1 x 10^6 units/mg.
Endotoxin:
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
Purity:
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Reconstitution:
Centrifuge vial prior to opening. Do not vortex. Reconstitute with H2O to 0.1-1.0 mg/ml.
Storage:
Before reconstitution: Short term: Room temperature; Long term: -20°C to -80°C. After reconstitution: Short term: 4°C; Long term: Aliquot and store with a carrier protein at -20°C to -80°C. Avoid freeze thaw cycles. Microcentrifuge before opening.
