Sequence:
MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL
Activity:
The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is <=0.1 ng/ml, corresponding to a specific activity of >=10^7 units/mg.
Endotoxin:
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
Purity:
Greater than 98% by SDS-PAGE and HPLC
Reconstitution:
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Storage:
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
