Storage:
Short-term storage at 4℃, some reagents should be kept at -20℃ when not in use for a long time.
Detection Method:
Colorimetric
Specificity:
This ELISA kit has excellent specificity for the detection of Nuclear Factor of Activated T-Cells, Cytoplasmic 1.
Format:
96-Well Microplate
Shipping Condition:
Ice packs
Standard Form:
Lyophilized
Sample Preparation:
Serum: Collect the sample in a serum separation tube and place the tube in a vertical position overnight at 4°C. Then centrifuge at approximately 1000 × g for 20 minutes. Analyze the serum immediately or aliquot and store at -20°C or -80°C.
Plasma: Use anticoagulant to collect plasma. Centrifuge for 15 minutes at 1000 × g within 30 minutes of collection. Assay immediately or aliquot and store at -20°C or -80°C. Avoid hemolysis and high cholesterol samples.
Tissue homogenates: Rinse tissues with ice-cold PBS to remove the excess of blood. Weigh the tissue first, homogenize the tissue on ice with a homogenizer, and then ultrasonically process the cell suspension. Centrifuge the homogenates at 5000 × g for 5 min and collect the supernatant. Assay immediately or aliquot and store at -20°C.
ELISA Kit Components:
Pre-coated 96-Well Microplate
Standard
Solution A
Solution B
Wash Buffer
Detection Solution A
Detection Solution B
Standard Diluent Buffer
TMB Substrate
Stop Solution
Assay Procedure Summary:
1. Prepare the reagents, standards, and samples needed for testing; 2. Aliquot 100 µl of diluted standard into the standard wells; 3. Aliquot 100 µl of standard diluent buffer into control (zero) well; 4. Add 100 µL/well sample to the microtiter plate, incubate 1 h at 37°C; 5. Then add 100 µL of the prepared detection solution A, incubate 1 h at 37°C; 6. After washing the plate 3 times, add 100 µL of the prepared detection solution B and incubate 90 min at 37°C; 7. After washing the plate 5 times, add 90 µL of substrate solution and incubate 10-20 min at 37°C; 8. Terminated by the addition of 50 µL stop solution, the absorbance was read at 450 nm by a microplate reader.
Precautions before Use:
This ELISA kit is not assembled, please assemble it before use.
Stop Solution:
The stop solution of this kit contains acid. Please pay attention to safe use and avoid direct contact.
