Warn:
The standard solution component of the ELISA kit contains a small amount of Tetracyclines, and the stop solution contains dilute sulfuric acid, please do not touch it directly.
Assay Type:
Direct competitive
ELISA Kit Principle:
The ELISA kit uses direct competition ELISA to quantitatively detect Tetracyclines. The antigen in the sample to be tested will compete with the tetracyclines-enzyme conjugate immobilized to the wells of the microtitrate plate for the binding sites of anti-tetracyclines antibodies. After incubation and washing at a suitable temperature and a certain period of time, then TMD is added for the color reaction and the result is measured at 450 nm. The intensity of the color in the microplate is negatively correlated with the concentration of the analyte.
Cross Activity:
The cross-reactivity of this ELISA kit for various congeners was Tetracycline (100%), Oxytetracycline (95%), 4-epi-tetracycline (95%), Demeclocycline (88%), Rolitetracycline (82%), Chlortetracycline (81%), 4-epi-oxytetracycline (71%), Methacycline (60%), Doxycycline (53%), 4-epi-chlortetracycline (29%), respectively.
Storage:
The ELISA kit should be stored at 2-8°C and should be performed at room temperature when used.
Notice:
In order to reduce the influence of various variables on the experimental results during the experiment, you must strictly control the experimental conditions, and the entire experimental operation process should be completed by the same person.
Precautions before Use:
Prepare the conjugate, substrate, buffer and stop solution. And before the test analysis, the reagents in the ELISA kit and the sample to be tested need to be incubated to room temperature.
Detection Method:
Colorimetric
Shipping Condition:
Ice packs
