Warn:
The stop solution contains dilute sulfuric acid, please do not touch it directly.
ELISA Kit Principle:
This ELISA kit uses sandwich ELISA to quantitatively detect Vitamin A. The polyclonal antibody specific for vitamin A is fixed on the microtiter plate, and then the sample to be tested is added. Wash after a certain period of incubation at a suitable temperature. Add the anti-vitamin-A monoclonal antibody conjugated to horseradish peroxidase (HRP), and then add TMD for color development. The color result is measured by an automatic plate reader at 450 nm.
Cross Activity:
This ELISA kit specifically detects Vitamin A and almost has no cross-reactivity to other compounds of similar structure.
Storage:
The ELISA kit should be stored at 2-8°C and should be performed at room temperature when used.
Notice:
In order to reduce the influence of various variables on the experimental results during the experiment, you must strictly control the experimental conditions, and the entire experimental operation process should be completed by the same person.
Precautions before Use:
Prepare the conjugate, substrate, buffer and stop solution. And before the test analysis, the reagents in the ELISA kit and the sample to be tested need to be incubated to room temperature.
Detection Method:
Colorimetric
Shipping Condition:
Ice packs
